Gene description for RPSA
Gene name ribosomal protein SA
Gene symbol RPSA
Other names/aliases 37LRP
67LR
ICAS
LAMBR
LAMR1
LBP
LBP/p40
LRP
LRP/LR
NEM/1CHD4
SA
lamR
p40
uS2
Species Homo sapiens
 Experiment description of studies that identified RPSA in Melanoma cells

1
Experiment ID453
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22635005   
OrganismHomo sapiens
Experiment descriptionMelanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
AuthorsPeinado H, Alecković M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, García-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D.
Journal name Nat Med
Publication year2012
SampleMelanoma cells
Sample nameB16-F10, SK-MEL-202, SK-MEL035, SK-MEL-265
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120019: EV-METRIC:44%, 44%, 44%

2
Experiment ID618
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameM14
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

3
Experiment ID986
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 25261472   
OrganismHomo sapiens
Experiment descriptionComplex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment.
AuthorsLiang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK.
Journal name J Biol Chem.
Publication year2014
SampleMelanoma cells
Sample nameSK-MEL-5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140188: EV-METRIC:22%

4
Experiment ID1627
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

5
Experiment ID1628
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

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